p66Shc-mediated oxidative stress is involved in gestational diabetes mellitus

Figure 4 Expression of dynamin-related protein 1 and reactive oxygen species in JEG3 cells after transfection with wt-p66Shc, si-p66Shc, and negative controls. A: mRNA expression of dynamin-related protein 1 (Drp1) in the wt-p66Shc and negative control (NC) cells was determined by quantitative real-time PCR; B: The mRNA expression of Drp1 in the si-p66Shc and NC groups was determined by qRT-PCR; C: The levels of ROS in the wt-p66Shc, si-p66Shc and NC groups were determined by diluted dihydroethidium staining. ^hP < 0.01 vs NC; ⁱP < 0.05 vs NC. wt-p66Shc: Transfected with plasmid DNA; si-p66Shc: Transfected with siRNA; NC: Negative control; ROS: Reactive oxygen species; Drp1: Dynamin-related protein 1.