CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes

Figure 3 Macrophage phagocytosis is increased by CD47 siRNA transfection while recovered with CD47 overexpression. A and B: Western blot analysis revealing CD47 relative protein expression with CD47 siRNA transfection and statistical data; C: Macrophage phagocytosis was enhanced when Min6 cells were transfected with CD47 siRNA; D and E: Western blot analysis indicating CD47 relative protein expression when transfection with CD47 open reading frame (ORF; CD47 overexpression) and statistical data; F: Macrophage phagocytosis was impaired by CD47 ORF transfection under STZ condition. GAPDH served as a loading control. Western blot analysis represents the results of three independent experiments. Student's ttest was performed. ^aP < 0.01 vs CTL. CTL: Control. CD47: Cluster of differentiation 47.