Maternal low protein diet induces persistent expression changes in metabolic genes in male rats

Figure 2 Expression of pyruvate dehydrogenase kinase 4 and citrate synthase mRNA in adipose tissue of rats. Rats were exposed to maternal low protein diet during gestation and lactation, and their RNA extracted from adipose tissue was analyzed by quantitative reverse transcription PCR. Data are shown as mean ± standard error means analyzed by two-way ANOVA with the mother’s diet (normal protein; low protein) and age (30 d, 90 d) as variable factors. Bonferroni’s post-hoc test was used. Differences between diet groups are indicated by an asterisks; differences between ages are indicated by bars. ^aP < 0.05, ^bP < 0.01. NP: Normal protein; LP: Low protein; PDK4: Pyruvate dehydrogenase kinase 4; CS: Citrate synthase.