Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 11 Influence of CL 316243, vanadate, amine oxidase inhibitors and antioxidants on glucose uptake in mouse adipocytes. Fat cells from wild-type mice were subjected to 2-deoxyglucose (2-DG) uptake in the presence of CL 316243, or catecholamines, insulin and vanadate. A: The β₃-adrenoceptors agonist was incubated alone (control, closed diamonds) or with vanadate (open squares) at the indicated concentrations; B: 1 mmol/L adrenaline (open columns) and noradrenaline (black columns) or 100 nmol/L insulin (shaded columns) were incubated with 100 µmol/L vanadate, without (control) or in the presence of pargyline 0.1 mmol/L, or pargyline + semicarbazide 1 mmol/L, or with 1 mmol/L of the antioxidants: N-acetylcysteine, glutathione, ascorbic acid. 2-DG uptake was expressed as the percentage of the insulin effect (which reached 1.73 ± 0.33 nmol 2-DG/100 mg lipids/0 min). The mean ± SE of 4 determination was determined. A significant difference from the respective control was observed at: ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. 2-DG: 2-Deoxyglucose.