Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 10 Influence of vanadate on hexose uptake into adipocytes from control and β-less mice. Suspensions of mouse fat cells from control (upper panel) or mice with triple KO for the β₁-, β₂-, β₃-adrenoceptors (lower panel) were incubated for 45 min without (basal, set at 0%), with 100 nmol/L insulin (set at 100%), or with the indicated doses of amines alone (open columns), or in combination with 100 µmol/L vanadate (dark columns), then [³H]-2-deoxyglucose uptake was assayed over 10 min. The mean ± SE of 4 (triple KO, lower panel) to 13 (control, upper panel) adipocyte preparations containing 17.2 ± 1.7 and 12.0 ± 1.6 mg lipids/400 µL, respectively, was determined. A significant difference from the corresponding condition without vanadate was observed at: ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.