Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 6 Dose-dependent activation by adrenaline and noradrenaline of hexose uptake in rat adipocytes: influence of the α₂-adrenoreceptors antagonist methoxy-idazoxan. Rat fat cells were incubated for 45 min with the indicated concentrations of adrenaline (left panel) or noradrenaline (right panel) without (control, squares), or with 0.01 mmol/L RX 821002 (triangles) prior to the 2-deoxyglucose uptake assay. Hexose transport was expressed as the fold increase relative to basal set at 1.0. The mean ± SE of 4 separate experiments performed in the presence of 100 µmol/L vanadate (orange arrow) was determined. No significant difference was found between the inhibitor and the respective control. 2-DG: 2-Deoxyglucose.