Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

Figure 4 Lack of influence of the dopaminergic antagonist metoclopramide on insulin- or amine-stimulated hexose uptake in rat adipocytes. Rat fat cells were incubated for 45 min with the indicated concentrations of agents without (control, closed columns), or with 0.1 mmol/L metoclopramide (open columns); then uptake of the glucose analogue 2-deoxyglucose (2-DG) was assayed over 10 min. 2-DG uptake was expressed as the fold increase relative to basal uptake set at 1.0. The mean ± SE of 4 separate experiments performed in the presence of 100 µmol/L vanadate (orange arrow) was determined. A significant difference between metoclopramide and the respective control was observed at: ^aP < 0.05. 2-DG: 2-Deoxyglucose.