MicroRNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6

Figure 4 The miR-320 family targets CDK6. A: Seven candidate target genes of miR-320a were identified as being common from the results of expression profiling and two bioinfomatics algorithms. The decreased expression of 497 pieces was observed in gene expression profiling of SW480 transfected with an miR-320a mimic and a mimic control (fold-change: < 0.67), and target genes of miR-320a were predicted by TargetScan and Pic tar; B: CDK6 has a putative miR-320 family-binding site mapped to the 3′ UTR; C and D: mRNA and protein levels of CDK6 in colon cancer cell lines (HT29 and SW480) transfected with miR-320 family mimics and a mimic control; E: The relative CDK6 expression level compared with that in non-neoplastic mucosa in 16 matched samples; F-J: Pearson’s correlation analysis between the expression levels of the miR-320 family and CDK6 in 16 matched samples (^aP < 0.05). UTR: Untranslated regions; 320am: miR-320a mimics; 320bm: miR-320b mimics; 320cm: miR-320c mimics; 320dm: miR-320d mimics; 320em: miR-320e mimics; mc: Mimic control; C: Carcinoma; A: Adenoma; N: Adjacent non-neoplastic mucosa.