Role of genetic detection in peritoneal washes with gastric carcinoma: The past, present and future

Table 1 Primer sequences used for reverse transcriptase polymerase chain reaction

Gene	Sequence of primer pair (5’→3’)	Tm (°C)	Amplicon length (bp)
CEA	F; TCTGGAACTTCTCCTGGTCTCTCAGCTGG	69	160
	R; TGTAGCTGTTGCAAATGCTTTAAGGAAGAAGC
CK-20	F; GGTCGCGACTACAGTGCATATTACA	72	121
	R; CCTCAGCAGCCAGTTTAGCATTATC
Tripsinogen	F; ACCACCATGAATCCACTCCTG	62
	R; GCTTTAGCTATTGGCAGCTAT
MMP-7	F; ATGTTAAACTCCCGCGTCATA	72	418
	R; CAGCATACAGGAAGTTAATCC
DDC	F; AAGCACAGCCATCAGGATTCA	70
	R; TGGACATGCTTGCGGATATAAG
L3PP	F; GATGCTGTGTGTTTTGAT GTTGAC	95
	R; CTTGACTTGTTGCCTGATCACATT
MAGE A1-6	F; CTGAAGGAGAAGATCTGCC	60	855
	R; CTCCAGGTAGTTTTCCTGCAC

Tm: Melting temperature; F: Forward; R: Reverse; CEA: Carcinoembryonic antigen; CK-20: Cytokeratin-20; MMP-7: Matrix metalloproteinase-7; DDC: Dopa decarboxylase; L3PP: L-3 phosphoserine phosphatase heparanase; MAGE: Melanoma associated gene.