Novel diet-related mouse model of colon cancer parallels human colon cancer

Figure 16 Lower regions of mouse colonic crypts immunostained for beta-catenin. Mice fed diets: A: Control; B: Diet + deoxycholic acid (DOC); C: Diet + DOC + chlorogenic acid (CGA). In A (control diet), in the stem cell region (lowest cells in the crypts), cells have beta-catenin expression localized to their membrane regions as shown by arrows. In B (diet + DOC), the stem cell region shows substantial nuclear localization of beta-catenin (arrows). In C (diet + DOC + CGA), stem cell region nuclei are largely deficient in beta-catenin, and the cytoplasm has low levels of beta-catenin, similar to the levels in mice fed the control diet alone. Images taken with 40× objective lens.