Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation

Figure 3 Radiosensitizing effects of autophagy inhibition by chloroquine. A: MTT assays in HCT-116 and HT-29 cell lines 72 h after treatment with CQ (10 μmol/L) and RT (2-8 Gy); B: Clonogenic survival of HCT-116 and HT-29 cells following treatment with CQ (0.1 and 0.5 μmol/L, respectively) and/or RT (2-8 Gy); C: Western blot in HT-29 cells harvested 24 h post-treatment with CQ (10 μmol/L) and/or RT (8 Gy); D: FACS analysis in HT-29 cells 24 h following treatment with CQ (10 μmol/L) and/or RT (8 Gy); E: Annexin V and PI staining in HT-29 cells 24 h following treatment with CQ (10 μmol/L) and/or RT (8 Gy). CQ: Chloroquine; RT: Radiation therapy.