Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation

Figure 2 Chemoradiation-induced autophagy. A: Western blot of LC3 in HT-29 cells harvested 24 h after treatment with RT (4 Gy), 5-FU (15 μmol/L) or 5-FU and RT; B: Western blot of LC3 in HT-29 cells harvested 24 h after treatment with RT (8 Gy), 5-FU (15 μmol/L) or 5-FU and RT; C: GFP-labeled LC3 fluorescence in HT-29 cells fixed 6 h after treatment with Rapamycin (200 nmol/L), 5-FU (15 μmol/L) or combination RT (2-8 Gy) and 5-FU (15 μmol/L). RT: Radiation therapy; 5-FU: 5-fluorouracil.