CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses

Figure 4 Influence of cagPAI status and CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs variations on interleukin-8 expression and cell elongation. CagPAI-negative strains (n = 6), cagPAI-defective strains (n = 24) and functional-cagPAI strains with either one (n = 40) or more than one (n = 20) EPIYA-C motifs were cocultured with AGS cells. (A) 6 h and (B) 30 h coculture supernatants were collected and assessed for interleukin-8 concentration by ELISA. Mean values are represented by horizontal lines within the scatterplots. Experiments for each strain were run in duplicates; (C) 6 h and (D) 24 h coculture photographs were taken and the percentage of hummingbird cells was determined. Mean values are represented by horizontal lines within the scatterplots. Experiments for each strain were run in duplicates. NS: Not significant.