CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses

Figure 2 Phosphorylation of CagA protein in AGS cells after coculture with Helicobacter pylori CagA-positive strains. Cell lysates were evaluated by western blot using anti-phosphotyrosine or anti-CagA antibodies. A representative assay is shown. Lane 1: 11 637 control strain with a functional cagPAI and 5 Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs (ABCCC); Lanes 2 and 6: Isolates with a defective cagPAI lacking expression of CagA; Lanes 7 and 8: Isolates with a defective cagPAI expressing CagA, with absence of CagA phosphorylation; Lanes 3-5: Isolates with a functional CagPAI with three (ABC; lane 3) or four (ABCC; lanes 4 and 5) EPIYA motifs; Lane 9: Uninfected AGS cells.