T(11;18)(q21;q21)-positive gastrointestinal MALT lymphomas are heterogeneous with respect to the VH gene mutation status

Table 5 Usage of the rearranged V_H, D_H and J_H gene segments and V_H mutation analysis of gastric MALT-type lymphoma, characterised by the presence or absence of the API2-MALT1 fusion abnormality

Case	API2-MALT1	Germline VH		Identity (%)	Obs R/S CDR	Obs R/S FR	Exp R/S CDR	Exp R/S FR	pCDR	pFR	DH	JH
		Locus	Name
1	+	VH3-30	DP-49	92.4	4/2	5/4	3/1	8/3	0.217	0.062	DH3-10	JH3
2	+	VH3	HHG4	97.7	1/2	0/1	1/0	2/1	0.421	-	NI	JH4
3	+	VH1-69	DP-10	85.3	11/3	11/4	7/2	15/5	0.030	0.040	DH1-1	JH5
4	+	VH4-34	DP-63	98.7	0/0	1/2	1/0	2/1	-	0.352	NI	JH6
5	+	VH4-39	DP-79	98.6	0/0	0/3	1/0	2/1	-	-	NI	JH4
61	+	VH3-33	DP-50	100	0/0	0/0	-	-	-	-	DH1-20	JH6
7	+	VH3-30	DP-49	95.1	5/0	2/2	2/1	5/2	0.030	0.048	DH3-10	JH4

¹Results obtained on the diagnostic intestinal biopsy as well as on a gastric biopsy after 54 mo follow-up; V_H genes: Immunoglobulin heavy chain variable gene; R: Replacement mutation; S: Silent mutation; CDR: Complementarity determining regions; FR: Framework regions; Obs R/S CDR and Obs R/S FR: Number of the observed R and S mutations in the CDR and FR, respectively; Exp R/S CDR and Exp R/S FR: Number of expected R and S mutations in the CDR and FR, respectively; pCDR and pFR: Probability that excess or scarcity of the R mutations in the V_H gene CDR or FR resulted from chance only; P values < 0.05 were considered statistical significant; NI: Not identified. V_H sequences were compared with germline V_H sequences included in the VBASE sequence directory.