Experience with peritoneal mesothelioma at the Milan National Cancer Institute

Figure 2 Representative Western blotting experiments illustrating survivin expression in STO cells exposed to oligofectamine alone or transfected with control siRNA and survivin siRNA. A: β-actin was used as a control for protein loading; B: Densitometric quantification of survivin band intensities in oligofectamine-exposed cells (empty column) and cells transfected with the control siRNA (gray column) or the survivin siRNA (black column). Data represent mean ± SD of 3 independent experiments. ^aP < 0.02; ^bP < 0.01; Student’s t test; such inhibition was highest (around 80%; P < 0.01) at 48 h and 72 h after transfection and still appreciable, although to a lesser extent (around 50%; P < 0.02), at 96 h.