Tetramethylpyrazine induces reactive oxygen species-based mitochondria-mediated apoptosis in colon cancer cells

Figure 2 N-acetyl-L-cysteine and Z-VAD-FMK inhibit tetramethylpyrazine-induced accumulation of intracellular reactive oxygen species. A: Intracellular reactive oxygen species (ROS) of SW480 and HCT116 cells treated with tetramethylpyrazine with or without Z-VAD-FMK and N-acetyl-L-cysteine detected by flow cytometry; B: Bar chart of intracellular ROS level in four groups of SW480 and HCT116 cells treated as indicated. ^aP < 0.001. TMP: Tetramethylpyrazine; NAC: N-acetyl-L-cysteine.