Detecting plasma SHOX2, HOXA9, SEPTIN9, and RASSF1A methylation and circulating cancer cells for cholangiocarcinoma clinical diagnosis and monitoring

Figure 6 Kaplan-Meier curves modeling the effect of methylation on overall survival in cholangiocarcinoma. Overall survival was calculated from plasma sampling time until death from any cause, and cases were censored at last follow-up. The effect of high and low methylation levels of SHOX2, RASSF1A, SEPTIN9, and HOXA9 were evaluated using a univariate approach with the log rank method. A: SHOX2; B: RASSF1A; C: SEPTIN9; D: HOXA9. Survival curves were estimated with the Kaplan-Meier method. Group definition: The cholangiocarcinoma samples were divided into high (DCtSHOX2 ≤ 9; DCtRASSF1A ≤ 12; DCtSEPTIN9 ≤ 9; DCtHOXA9 ≤ 8) and no/low methylation levels (DCtSHOX2 > 9; DCtRASSF1A > 12; DCtSEPTIN9 > 9; DCtHOXA9 > 8). HR: Hazard ratio.