Nitric oxide synthase 1 inhibits the progression of esophageal cancer through interacting with nitric oxide synthase 1 adaptor protein

Figure 7 Nitric oxide synthase 1 and nitric oxide synthase 1 adaptor protein affect the level of cell autophagy and apoptosis. A and B: Western blotting detected the changes in the expression of autophagy-related proteins LC3B and P62 in TE-15 and OE-19 cell lines; C: Flow cytometry analyzed the level of apoptosis; D: TUNEL staining analyzed the level of apoptosis; E: 5-Ethynyl-2’-deoxyuridine assay analyzed the cell proliferation ability; F and G: Immunohistochemistry analysis of P62, LC3B expression in mouse tumor tissues. Values are mean ± SD. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. n = 3 experiments. EDU: 5-Ethynyl-2’-deoxyuridine; oe-NOS1: Overexpression of nitric oxide synthase 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; si-NOS1AP: Silencing of nitric oxide synthase 1 adaptor protein.