Nitric oxide synthase 1 inhibits the progression of esophageal cancer through interacting with nitric oxide synthase 1 adaptor protein

Figure 2 Effect of nitric oxide synthase 1 on esophageal cancer cell function. A: Quantitative real-time polymerase chain reaction to detect the expression of nitric oxide synthase 1 (NOS1) in Het-1A, KYSE-70, TE-9, TE-15, OE-19 cells; B: Western Blotting to detect the expression of NOS1 in Het-1A, KYSE-70, TE-9, TE-15, OE-19 cells.; C: Cell counting kit-8 to detect cell proliferation and viability; D: Flow cytometry to analyze the effect of NOS1 on cell apoptosis; E: Effect of NOS1 on cell invasion ability; F: Effect of NOS1 on cell migration ability; G: Effect of NOS1 on the cell cycle. Values are mean ± SD. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. n = 3 experiments. NOS1: Nitric oxide synthase 1; oe-NOS1: Overexpression of nitric oxide synthase 1; OD: Optical density.