F-box protein 22: A prognostic biomarker for colon cancer associated with immune infiltration and chemotherapy resistance

Figure 7 Knockdown of the expression of F-box protein 22 inhibits proliferation, and migration of colon cancer cells in vitro. A: The transfection efficiency of si-F-box protein 22 (si-FBXO22) in the HCT116 cell lines and DLD1 cell lines were detected by polymerase chain reaction; B: The transfection efficiency of si-FBXO22 in the HCT116 cell lines and DLD1 cell lines were detected by western blotting; C and D: Cell Counting Kit-8 assay and 5-ethynyl-2’-deoxyuridine assay were applied to detect the efficiency of FBXO22 knockdown on the proliferation of HCT116 and DLD1 cell lines; E and F: Transwell migration assay and Wound-healing assay were applied to detect the efficiency of FBXO22 knockdown on the migration of HCT116 and DLD1 cell lines. ^aP < 0.01 vs blank group; ^bP < 0.001 vs blank group; ^cP < 0.001 vs negative control group; ^dP < 0.01 vs negative control group. FBXO22: F-box protein 22; NC: Negative control.