Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis

Figure 6 O6-methylguanine DNA methyltransferase inhibited rectal cancer progression by reducing protein tyrosine phosphatase nonreceptor type 4 expression. A and B: Protein tyrosine phosphatase nonreceptor type 4 (PTPN4) protein levels in SW837 cells were assessed through Western blot analysis; C: SW837 cell proliferation detected using Cell Counting kit-8 assay; D and E: Transwell assay performed used to assess SW837 cell invasion; F and G: SW837 cell apoptosis was examined using flow cytometry; H and I: E-cadherin, N-cadherin and vimentin levels in SW837 cells were measured using Western blot analysis. Data are presented as mean ± SE. ^aP < 0.05; ^bP < 0.01; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MGMT: O6-methylguanine DNA methyltransferase; PTPN4: Protein tyrosine phosphatase nonreceptor type 4; sh-MGMT: Short hairpin RNAs targeting O6-methylguanine DNA methyltransferase; sh-PTPN4: Short hairpin RNAs targeting protein tyrosine phosphatase nonreceptor type 4.