Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis

Figure 3 Centromere protein A promoted DNA methyltransferase 1-mediated O6-methylguanine DNA methyltransferase methylation. A: Schematic diagram for screening interacting proteins of centromere protein A (CENPA); B: DNA methyltransferase 1 (DNMT1) has correlation with CENPA in rectal cancer as shown by the Gene Expression Profiling Interactive Analysis (GEPIA) database; C: Interaction between CENPA and DNMT1 verified using co-immunoprecipitation assay; D: CpG islands within the methyltransferase (MGMT) promoter region; E: DNMT1 enrichment of the MGMT promoter region examined through chromatin immunoprecipitation assay; F: Methylation-specific PCR was used to examine MGMT methylation level in SW837 cells; G: MGMT mRNA levels were measured using RT-qPCR; H: MGMT protein levels were measured using Western blot analysis. Data are presented as mean ± SE. ^aP < 0.05; ^bP < 0.01; U: Unmethylated; M: Methylated; CENPA: Centromere protein A; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; DNMT1: DNA methyltransferase 1; MGMT: O6-methylguanine DNA methyltransferase; sh-CENPA: Short hairpin RNAs targeting Centromere protein A.