MEX3A promotes cell proliferation by regulating the RORA/β-catenin pathway in hepatocellular carcinoma

Figure 8 Role of RORA in hepatocellular carcinoma as a downstream gene for MEX3A. A-C: Real-time fluorescent quantitative PCR and western blotting detection of the knockdown efficiency of MEX3A and RORA; D: Cell counting kit-8 assay detection of the proliferative ability of the cells divided into four groups of HepG2 cells (“c”represents significant difference between the shNC + siNC and shMEX3A + siNC groups; “d” represents significant difference between the shMEX3A + siRORA and shMEX3A + siNC groups); E and F: Crystal violet staining was used to detect the clone formation ability of the cells divided into four groups; G and H: Western blotting detection of the effect of MEX3A or RORA silencing on expression of proteins in the Wnt/-catenin pathway. Data are presented as the mean ± SD; I: Immunofluorescence was used to detect the accumulation of β-catenin in the nucleus. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.001. Scale bar = 50 μm.