Chlorogenic acid induces hepatocellular carcinoma cell ferroptosis via PTGS2/AKR1C3/GPX4 axis-mediated reprogramming of arachidonic acid metabolism

Figure 3 Acquisition, expression difference, and prognostic analysis of chlorogenic acid intervention targets (mean ± SD, n = 374). A: Volcano plot of differential analysis based on GSE19665 dataset; B: Volcano plot of differential analysis based on GSE84402 dataset; C: Venn diagram showing the intersection of chlorogenic acid (CGA) targets, hepatocellular carcinoma (HCC) targets from GSE19665 and GSE84402 datasets, and ferroptosis-related genes; D: Unpaired expression analysis of CGA intervention targets in HCC patients; F: Paired expression analysis of CGA intervention targets in HCC patients; E and G: Lasso regression analysis of CGA intervention targets; H: Immunohistochemical analysis of CGA intervention targets in the Human Protein Atlas database. Not sig: Not significant; FRG: Ferroptosis-related genes; TPM: Transcripts per million; AKR1C3: Aldo-keto reductase family 1 member C3; AURKA: Aurora kinase A; AR: Androgen receptor.