DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

Figure 6 SERPINH1 knockdown attenuates the effects of DNMT1 on gastric cancer cell proliferation, migration, invasion and epithelial mesenchymal transition. A: Western blot detection of SERPINH1 transfection efficiency in HGC27 cells; B: Western blot detection of SERPINH1 expression in HGC27 cells; C: Proliferation activity of HGC27 cells detected by cell counting kit 8 assay; D: HGC27 cell migration detected by scratch experiments; E: HGC27 cell invasion detected by Transwell assay; F: Expression of the epithelial mesenchymal transition-related proteins E-cadherin, N-cadherin and vimentin in HGC27 cells, as detected by Western blot; G: Expression of the epithelial mesenchymal transition-related protein vimentin in HGC27 cells detected by immunofluorescence (scale bar = 10 μm). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. OE-NC: Overexpression negative control; OE-DNMT1: DNMT1 overexpression; si-NC: Small interfering-negative control; si-SERPINH1: Small interfering SERPINH1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; OD: Optical density; DAPI: 4’,6-diamidino-2-phenylindole.