DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

Figure 5 DNMT1 regulates SERPINH1 expression through microRNA-125a-5p. A: Positive expression of SERPINH1 in clinical samples detected by immunohistochemistry (scale bar = 50 μm); B: Western blot detection of SERPINH1 expression in cells; C: A bioinformatics platform (http://starbase.sysu.edu.cn/) was used to predict the target binding site of microRNA (miR)-125a-5p; D: Real-time polymerase chain reaction (RT-qPCR) verification of the targeted binding of miR-125a-5p and SERPINE1; E: RT-qPCR was used to detect the efficiency of miR-125a-5p knockdown in HGC27 cells; F: Western blot detection of SERPINH1 expression in HGC27 cells; G: RT-qPCR detection of miR-125a-5p expression in HGC27 cells; H: Western blot detection of SERPINH1 expression in HGC27 cells. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. WT: Wild-type; MUT: Mutant; NC: Negative control; miR: MicroRNA; GC: Gastric cancer; OE-NC: Overexpression negative control; OE-DNMT1: DNMT1 overexpression.