DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

Figure 4 DNMT1 affects gastric cancer cell proliferation, migration, invasion and epithelial mesenchymal transition by mediating microRNA-125a-5p methylation. A: DNMT1 expression in HGC27 cells detected by Western blot; B: Methylation-specific polymerase chain reaction detection of the microRNA (miR)-125a-5p promoter methylation level in HGC27 cells; C: MiR-125a-5p expression in HGC27 cells detected by real-time polymerase chain reaction; D: Detection of HGC27 cell proliferation by cell counting kit 8 assay; E: HGC27 cell migration detected by scratch test; F: HGC27 cell invasion determined by Transwell assay; G: Western blot detection of the expression of the epithelial mesenchymal transition-related proteins E-cadherin, N-cadherin and vimentin in HGC27 cells; H: Immunofluorescence detection of the expression of the epithelial mesenchymal transition-related protein vimentin in HGC27 cells (scale bar = 10 μm). ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. OE-NC: Overexpression negative control; OE-DNMT1: DNMT1 overexpression; 5-aza-dC: 5-aza-2’-deoxycytidine; miR: MicroRNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; OD: Optical density; M: Methylation; U: Unmethylated; DAPI: 4’,6-diamidino-2-phenylindole; PBS: Phosphate-buffered saline.