DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

Figure 3 DNMT1 represses microRNA-125a-5p expression through methylation. A: DNMT1 expression in clinical samples detected by real-time polymerase chain reaction (RT-qPCR); B: Positive expression of DNMT1 in clinical samples detected by immunohistochemistry (scale bar = 50 μm); C: Western blot analysis of DNMT1 expression in HGC27 cells; D: Toxicity of 5-aza-2’-deoxycytidine in HGC27 cells; E: RT-qPCR detection of microRNA (miR)-125a-5p expression in HGC27 cells after treatment with different concentrations of 5-aza-2’-deoxycytidine; F: Western blot detection of DNMT1 transfection efficiency in HGC27 cells; G: Methylation of miR-125a-5p in HGC27 cells detected by methylation-specific polymerase chain reaction; H: RT-qPCR detection of miR-125a-5p expression in HGC27 cells. ^cP < 0.001. OE-NC: Overexpression negative control; OE-DNMT1: DNMT1 overexpression; si-NC: Small interfering-negative control; si-DNMT1: Small interfering DNMT1; 5-aza-dC: 5-aza-2’-deoxycytidine; miR: MicroRNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GC: Gastric cancer; M: Methylation; U: Unmethylated.