DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

Figure 2 Overexpression of microRNA-125a-5p inhibits gastric cancer cell proliferation, migration, invasion and epithelial mesenchymal transition. A: Transfection efficiency of the microRNA (miR)-125a-5p mimic in HGC27 cells, as detected by real-time polymerase chain reaction (RT-qPCR); B: Proliferation of HGC27 cells detected by cell counting kit 8 assay; C: Migration of HGC27 cells detected by scratch experiments; D: HGC27 cell invasion detected by Transwell assays; E: Western blot detection of the expression of the epithelial mesenchymal transition (EMT)-related proteins E-cadherin, N-cadherin and vimentin in HGC27 cells; F: Immunofluorescence detection of the expression of the EMT-related protein vimentin in HGC27 cells (scale bar = 10 μm). ^bP < 0.01. ^cP < 0.001. EMT: Epithelial mesenchymal transition; NC: Normal control; miR: MicroRNA; OD: Optical density; DAPI: 4’,6-diamidino-2-phenylindole; miR: MicroRNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.