Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

Figure 7 Short hairpin RNA targeting transmembrane protein 176B inhibits the epithelial-mesenchymal transition process via the NLRP3 signaling pathway in CT26 cells. A: Enzyme-linked immunosorbent assay was used to quantify the expression of the interleukin-18 (IL-18) in CT26 cells; B and C: Western blot was used to measure the expression of the NLRP3, ASC, caspase-1, IL-18, TMEM176B, p-p65, p65, MMP-9, transforming growth factor-beta, N-cadherin, E-cadherin, Vimentin in CT26 cells. ^aP <0.05; ^bP <0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Overexpression-negative control; IL-18: Interleukin-18; TGF-β: Transforming growth factor-beta.