Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

Figure 6 Short hairpin RNA targeting transmembrane protein 176B inhibits migration and invasion in CT26 cells co-cultured with activated natural killer cells while promoting cell apoptosis. A-C: Wound healing and Transwell assays were used to assess migration distance, migration and invasion rates of CT26 cells co-cultured with interleukin-2 (IL-2)-exposed natural killer (NK) cells for 12 hours. With magnifications of 200 × (scale bar 100 μm) and 40 × (scale bar 400 μm). n = 3 in each group; D: Flow cytometry was used to analyze the apoptosis rate of CT26 cells co-cultured with IL-2-exposed NK cells. n = 3 in each group. ^aP < 0.05; ^bP < 0.01. sh-TMEM176B: Short hairpin RNA targeting transmembrane protein 176B; sh-NC: Short hairpin RNA interference-negative control; NK: Natural killer; IL-2: Interleukin-2.