To investigate the effect and mechanism of tetrahydrocurcumin on hepatocellular carcinoma based on phosphoinositide 3-kinases/AKT signaling pathway

Figure 11  Effects of tetrahydrocurcumin on the expression of phosphoinositide 3-kinases-AKT signaling pathway-related proteins in HepG2 and Huh7 cells. A: Western blot detection of core proteins related to phosphoinositide 3-kinases (PI3K)/AKT signaling. HepG2 and Huh7 cells were treated with THC (25, 50, and 100 μM) for 48 hours. Representative bands of PI3K, p-PI3K, AKT, p-AKT, p53, caspase-3, B cell leukemia/lymphoma 2 associated X, and B cell leukemia/lymphoma 2 protein expression analyzed by Western blotting; B: Western blot analysis of the expression levels of core PI3K/AKT proteins in HepG2 and Huh7 cells after drug treatment. All data are presented as mean ± SD, n = 3. Compared to the control group, ns indicates no significance, ^aP < 0.05 vs the control group, ^bP < 0.01 vs the control group. PI3K: Phosphoinositide 3-kinases; Bax: B cell leukemia/lymphoma 2 associated X; Bcl-2: B cell leukemia/lymphoma 2.