To investigate the effect and mechanism of tetrahydrocurcumin on hepatocellular carcinoma based on phosphoinositide 3-kinases/AKT signaling pathway

Figure 9 The effect of tetrahydrocurcumin on the migration and invasion of HepG2 and Huh7 cells. A: Wound-healing assay reflecting the cell migration ability of HepG2 and Huh7 cells after tetrahydrocurcumin (THC, 25, 50, and 100 μmol/L) treatment for 48 hours and graph showing the wound healing rate of HepG2 and Huh7 cells after THC (25, 50, and 100 μmol/L) treatment for 48 hours; B: Transwell assays validated the effect of THC (25, 50, and 100 μmol/L) on the migration ability of HepG2 cells. The number of cells passing through the cell chamber was counted for statistical analysis after 48 hours of drug treatment, and the effect of the drug on cell invasion ability was also verified using matrigel. The number of cells passing through the cell chamber was counted for statistical analysis after 48 hours of drug treatment; C: Transwell experiment assessing the effect of THC (25, 50, and 100 μmol/L) on the migration ability of Huh7 cells. The number of cells passing through the cell chamber was statistically analyzed after 48 hours of drug treatment. The effect of the drug on cell invasion was also verified using matrigel. The number of cells passing through the cell chamber was analyzed after 48 hours of drug treatment. Scale bars: 200 μm. All data are expressed as mean ± SD, n = 3. ^bP < 0.01 vs the control group. THC: Tetrahydrocurcumin.