IL-22/IL-22R1 pathway enhances cholangiocarcinoma progression via ERK1/2 activation

Figure 3 IL-22 can resist apoptosis induced by nutrient deprivation and promote the migration and invasion of cholangiocarcinoma cells. A: IL-22 (200 ng/mL) was applied to HuCCT1 and RBE cells for 48 hours, along with cells that were not treated with IL-22, followed by an overnight starvation. Flow cytometry was then used to detect the level of apoptosis and to perform quantitative analysis of the results; B: IL-22 (200ng/mL) was applied to HuCCT1 and RBE cells for 48 hours, along with cells that were not treated with IL-22, followed by an overnight starvation. Immunofluorescence TUNEL assay was employed to assess the level of apoptosis and to conduct quantitative analysis of the results; C: Transwell experiments were conducted to assess the changes in migration and invasion capabilities of HuCCT1 and RBE cells following IL-22(200 ng/mL) treatment, along with quantitative analysis of the results; D: Wound healing assays were performed to evaluate the migration ability of HuCCT1 and RBE cells treated with IL-22 (200 ng/mL), along with quantitative analysis of the results. ^aP < 0.05, ^bP < 0.01. NC: Negative control.