IL-22/IL-22R1 pathway enhances cholangiocarcinoma progression via ERK1/2 activation

Figure 2 IL-22 promotes the proliferation of cholangiocarcinoma cells. A: CCK8 proliferation curves of HuCCT1 cells stimulated with different concentrations of IL-22 at various time points; B: CCK8 proliferation curves of RBE cells stimulated with different concentrations of IL-22 at various time points; C: Flow cytometry cell cycle profiles of HuCCT1 cells after stimulation with different concentrations of IL-22 for 48 hours; D: Quantitative analysis results of the cell cycle assessment by flow cytometry; E: Flow cytometry cell cycle profiles of RBE cells after stimulation with different concentrations of IL-22 for 48 hours; F: Quantitative analysis results of the cell cycle assessment by flow cytometry; G and H: Colony formation and quantitative analysis results of HuCCT1 and RBE cells after stimulation with IL-22 at a concentration of 200 ng/mL; I and J: EdU incorporation and quantitative analysis results of HuCCT1 and RBE cells after stimulation with IL-22 at a concentration of 200 ng/mL. ^aP < 0.05, ^bP < 0.01. NC: Negative control.