Tetrahydrocurcumin-induced apoptosis of hepatocellular carcinoma cells involves the TP53 signaling pathway, as determined by network pharmacology

Figure 10  Effect of tetrahydrocurcumin on the expression of P53 signaling pathway-related proteins in hepatocellular carcinoma cells. A: Western blotting analysis of core P53 signaling pathway-related proteins. HepG2 and Huh7 cells were treated with tetrahydrocurcumin (25, 50, or 100 μM) for 48 hours. Representative blots of P53, caspase-3, Bax, and Bcl-2 protein expression; B: Western blotting analysis of the expression of core proteins related to the P53 signaling pathway in hepatocellular carcinoma cells after drug treatment. All data are presented as the mean ± SD, n = 3. ^aP < 0.05; ^bP < 0.01; THC: Tetrahydrocurcumin.