LncRNA PCAT6 promotes progression and metastasis of colonic neuroendocrine carcinoma via MAPK pathway

Figure 6 Prostate cancer associated transcript 6 promotes the proliferation, migration, invasion, and epithelial-mesenchymal transition of colonic neuroendocrine carcinomas cells by targeting miR-326. A: The knockdown efficiency of miR-326 inhibitor in LCC-18 cells was confirmed by RT-qPCR; B: CCK-8 assays of cell viability; C: Colony formation assays of cell proliferation; C: Transwell assays of cell invasion; E: Wound healing assays of cell migration; F: The protein levels of E-cadherin, N-cadherin, Snail, and vimentin were measured by western blotting; G: The mRNA levels of E-cadherin, N-cadherin, Snail, and vimentin were assessed by RT-qPCR; H: The protein levels of p38, p-p38, ERK, and p-ERK were measured by western blotting. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs NC inhibitor, sh-NC, or vector group; ^dP < 0.01, ^eP < 0.001 vs sh-PCAT6 group. PCAT6: Prostate cancer associated transcript 6.