LncRNA PCAT6 promotes progression and metastasis of colonic neuroendocrine carcinoma via MAPK pathway

Figure 5 MiR-326 is a target of prostate cancer associated transcript 6. A: Subcellular distribution of prostate cancer associated transcript 6 (PCAT6) in LCC-18 cells; B: The candidate downstream miRNAs of PCAT6 were predicted by the TargetScan database; C: The miRNA levels of these candidate miRNAs in LCC-18 cells transfected with sh-NC or sh-PCAT6 were measured by real-time quantitative polymerase chain reaction (RT-qPCR); D: The overexpression efficiency of miR-326 mimics in LCC-18 cells was detected by RT-qPCR; E: The interaction between miR-326 and PCAT6 was determined by luciferase reporter assays; F: The level of miR-326 in tumor and adjacent nontumor samples collected from patients with NEC was assessed by RT-qPCR; G: The correlation between PCAT6 expression and miR-326 expression was determined by Pearson’s analysis. ^aP < 0.001. PCAT6: Prostate cancer associated transcript 6.