LncRNA PCAT6 promotes progression and metastasis of colonic neuroendocrine carcinoma via MAPK pathway

Figure 4 Prostate cancer associated transcript 6 enhances the epithelial-mesenchymal transition in neuroendocrine carcinomas. A and B: The protein and mRNA levels of E-cadherin, N-cadherin, Snail and GAPDH in LCC-18 cells were measured by western blotting and real-time quantitative polymerase chain reaction; C: The protein levels of vimentin, Snail and E-cadherin in neuroendocrine carcinomas tumor samples; D: The protein levels of p38, p-p38, ERK, and p-ERK in LCC-18 cells were evaluated by western blotting; E: The protein levels of p-ERK, E-cadherin, and vimentin in LCC-18 cells treated with or without U0126 (an ERK inhibitor) were measured by western blotting; F: The protein levels of p-p38, E-cadherin, and vimentin in LCC-18 cells treated with or without SB202190 (a p38 inhibitor) were detected by western blotting. ^aP < 0.001 vs vector group or PCAT6 group; ^bP < 0.001 vs sh-NC group. PCAT6: Prostate cancer associated transcript 6.