BIBR1532 inhibits proliferation and metastasis of esophageal squamous cancer cells by inducing telomere dysregulation

Figure 6 Effect of BIBR1532 on shelterin protein and key proteins in the DNA damage response pathway expression in KYSE150 and KYSE410 cells. A and B: Expression of telomeric-repeat binding factor 1 (TRF1), TRF2, TIN2-interacting protein 1, protection of telomeres 1, TIN2, and repressor activation protein 1 in KYSE150 (A) and KYSE410 (B) cells after treatment with indicated concentrations of BIBR1532 for 48 hours; C and D: Expression of γ-H2AX, p-ATM, CHK2, p-ATR, and CHK1 in KYSE150 (C) and KYSE410 (D) cells after treatment with the indicated concentrations of BIBR1532 for 48 hours. TRF1: Telomeric-repeat binding factor 1; TRF2: Telomeric-repeat binding factor 2; TPP1: TIN2-interacting protein 1; POT1: Protection of telomeres 1; TIN2: TRF1-interacting nuclear protein 2; RAP1: Repressor activation protein 1; γ-H2AX: Phosphorylated histone H2AX; p-ATM: Phosphorylated ataxia-telangiectasia mutated gene; p-ATR: Phosphorylated ataxia telangiectasia and Rad3-related protein; CHK2: Check point kinase 2; CHK1: Check point kinase 1.