Resveratrol inhibits pancreatic cancer proliferation and metastasis by depleting senescent tumor-associated fibroblasts

Figure 2 Stimulation with 3CKs induces senescence in cancer-associated fibroblasts. A: Primary cultures of cancer-associated fibroblasts (CAFs) isolated from pancreatic tumor tissue were established; B: The isolated and cultured primary cells showed strong expression of α-SMA; C: Stimulation with 3CKs inhibited the growth of CAFs; D: The growth rate of CAFs significantly decreased after stimulation with 3CKs; E-G: The expression of p21 and p16 significantly increased after stimulating CAFs with 3CKs; H: The depletion of Lamin B1 was observed in 3CKs-treated CAFs; I-P: Treatment with 3CKs induced upregulation of senescence-associated secretory phenotype factors. The scale bar represents 20 µm. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.