Network pharmacology study and in vitro experimental validation of Xiaojianzhong decoction against gastric cancer

Figure 7 Effect of Xiaojianzhong decoction on phenotype of gastric cancer cells. A-C: Viability of AGS, HGC-27, and GES-1 cells determined by CCK8 assay after Xiaojianzhong decoction (XJZ) treatment for 12, 24 and 36 h; D and E: Effect of XJZ on apoptosis of AGS cells detected by flow cytometry; F and G: Effect of XJZ on apoptosis of HGC-27 cells detected by flow cytometry; H and I: Effect of XJZ on cell cycle progression of AGS cells detected by flow cytometry; J and K: Effect of XJZ on cell cycle progression of HGC-27 GC detected by flow cytometry; L and M: Effects of XJZ on clone-forming ability of AGS cells; N and O: Effects of XJZ on clone-forming ability of HGC-27 cells. All experiments were repeated three times and the data are expressed as the mean ± SD, ^bP < 0.01, ^cP < 0.001; XJZ: Xiaojianzhong decoction; GC: Gastric cancer; NC: Negative control.