Lecithin-cholesterol acyltransferase is a potential tumor suppressor and predictive marker for hepatocellular carcinoma metastasis

Figure 5 DNA methyltransferase 1 regulates the expression of lecithin-cholesterol acyltransferase via affecting its methylation. A: Regression analysis between gene expression and DNA methylation of lecithin-cholesterol acyltransferase (LCAT); B-E: Regression analysis between DNA methylation of LCAT and the DNA methyltransferases DNMT1, UHRF1, DNMT3A and DNMT3B; F-H: Regression analysis between DNA methylation of LCAT and the DNA demethyltransferases TET1, TET2 and TET3; I-O: Regression analysis between DNA methyltransferases or demethyltransferases and LCAT; P-S: Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of mRNA expression levels of LCAT, DNMT1, DNMT3A and DNMT3B in HepG2, PLC/PRF5, Hep3B, and Huh7 cells; T-W: qRT-PCR analysis of LCAT mRNA after treatment with decitabine for 72 h in different HCC lines; X and Y: qRT-PCR analysis of mRNA expression levels of LCAT and DNMT1 in PLC/PRF5 and Hep3B. ^aP < 0.01, ^cP < 0.05, ^dP < 0.001. LCAT: Lecithin-cholesterol acyltransferase.