Effect of acacetin on inhibition of apoptosis in Helicobacter pylori-infected gastric epithelial cell line

Figure 5 Acacetin extract inhibits Helicobacter pylori-induced apoptosis in GES-1 cells. A and B: Protein levels of cleaved caspase-3, Bcl-2, and Bax were analyzed by immunoblotting following pre-treatment with acacia extract (10 µmol/L, 20 µmol/L) and subsequent stimulation with Helicobacter pylori(H. pylori) (cell to H. pylori ratio of 1:100) for 24 h. GAPDH served as an internal control, with the ratio in the blank control group set to 1; C and D: Apoptosis levels in the blank group, H. pylori-infected group, and H. pylori plus acacetin extract group were measured by flow cytometry; E: Representative images of cells stained with TUNEL and DAPI. × 40 magnification; F: Percentage of apoptotic cells quantified as the apoptosis index.