Insulin-like growth factor 2 targets IGF1R signaling transduction to facilitate metastasis and imatinib resistance in gastrointestinal stromal tumors

Figure 2 Insulin-like growth factor 2 promotes malignant characteristics and metastasis of gastrointestinal stromal tumors. A: Western blot measured the transfection efficiency of OE-insulin-like growth factor 2 (IGF2) or sh-IGF2 in gastrointestinal stromal tumors (GIST) 882 and GIST-T1 cells; B: ELISA of IGF2 expression in OE-IGF2 or sh-IGF2 transfected GIST882 and GIST-T1 cells; C: Cell counting kit-8 assay assessed cell viability in GIST882 and GIST-T1 cells; D: Transwell assay evaluated the migration of OE-IGF2- or sh-IGF2-transfected cells (scar bar = 50 μm); E: Transwell assays of the invasiveness of OE-IGF2 or sh-IGF2 transfected cells. (scar bar = 50 μm); F: Detection of proteins involved in epithelial-mesenchymal transition (vimentin, N-cadherin, E-cadherin, Twist1) in OE-IGF2 or sh-IGF2 transfected cells; G: Liver tissue from tumor xenografts in nude mice injected withOE-IGF2 transfected GIST-T1 cells; H: Liver metastasis determined by hematoxylin-eosin staining. Data are mean ± standard deviation. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001.