Expression and significant roles of the long non-coding RNA CASC19/miR-491-5p/HMGA2 axis in the development of gastric cancer

Figure 5 The regulatory relationship between CASC19, miR-491-5p and HMGA2 in vitro. A: The CASC19 expression of pc-CASC19 (over-expression vector) in HGC27 cell lines; B: The relative inhibition of luciferase expression of the miR-491-5p containing HMGA2 3’UTR co-transfected with pc-CASC19 or pc-NC in 293T cells; C and D: The expression of HMGA2 detected by quantitative reverse transcription polymerase chain reaction and western blot, MGC803 and AGS were transfected with si-NC, si-CASC19-2, co-transfected with si-CASC19-2 and miR-491 inhibitor, respectively, the HGC27 cells were transfected with pc-NC, pc-CASC19, co-transfected with pc-CASC19 and miR-491 mimics, respectively. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 was compared with the control group respectively. P < 0.05 was considered statistically significant.