Microvascular structural changes in esophageal squamous cell carcinoma pathology according to intrapapillary capillary loop types under magnifying endoscopy

Figure 2 Pathological data collection using Case Viewer software. A: Pathological slide stained with hematoxylin and eosin (magnification: × 100); B: Pathological slide stained for CD34 (magnification: × 100); C: Pathological slide stained for D2-40 (magnification: × 100); D: Measurement of intrapapillary capillary loop (IPCL)-related characteristics (red A: Apical distance from the vertex of the IPCL to the basement membrane; red B: Thickness of the epithelial layer); E: Enlarged orange region in picture D (red C: Short caliber of the IPCL; red D: Long caliber of the IPCL; and the region enclosed by the blue line is the area of the IPCL); F: Pathological slide stained for CD34 (magnification: × 800); the transverse diameter of each field of view was 719.8 μm; G: Pathological slide stained for CD34 (magnification: × 2000); the transverse diameter of each field of view is 287.9 μm.