N6-methyladenosine modification of hypoxia-inducible factor-1α regulates Helicobacter pylori-associated gastric cancer via the PI3K/AKT pathway

Figure 6 Knockdown of methyltransferase-like protein 14 cells promotes the activation of PI3K/AKT pathway and gastric cancer progression. A and B: Gastric epithelial cells transiently transfected with methyltransferase-like protein 14 small interfering RNA (siRNA) were infected with Helicobacter pylori, and then the protein levels of PI3K/AKT pathway proteins, epithelial-mesenchymal transition biomarkers, and invasion indicators (MMP2 and MMP9), were detected; C: Cell viability was assessed by cell counting kit-8 assay; D and E: Cell migration was detected by wound healing assay. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, compared with Hp27 + siRNA-negative control group. METTL14: Methyltransferase-like protein 14; NC: Negative control.