N6-methyladenosine modification of hypoxia-inducible factor-1α regulates Helicobacter pylori-associated gastric cancer via the PI3K/AKT pathway

Figure 5 Overexpression of methyltransferase-like protein 14 inhibits the activation of PI3K/AKT pathway and gastric cancer progression. A and B: After methyltransferase-like protein 14 (METTL14) overexpression, the related protein levels were checked by Western blot, including METTL14, p-PI3K, PI3K, p-AKT, AKT, N-cadherin, Vimentin, ZEB1, MMP2, and MMP9, all normalized to β-actin; C: Cell viability was detected by cell counting kit-8 assay; D and E: Cell migration was detected by wound healing assay. ^aP < 0.05, ^bP < 0.01, compared with Hp27 + pcDNA3.1(+) group. METTL14: Methyltransferase-like protein 14.